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Arecoline enhances the migratory and invasive potential of LAMC2 + OSCC cells. (A) Representative immunohistochemical staining images showing LAMC2 expression in OSCC tissues from betel nut chewers and nonchewers. The box‐and‐whisker plot on the right summarizes LAMC2 expression levels; each dot represents an individual clinical specimen (**** p < 0.0001 by Student's t ‐test). (B) Western blot images showing the expression of LAMC2, EMT‐associated markers (N‐cadherin, E‐cadherin, and Vimentin), and GAPDH in OSCC cell lines <t>SCC25</t> and SCC9 under control and arecoline‐treated conditions. (C) Representative Transwell assay images illustrating migratory and invasive capacities of SCC25 and SCC9 cells in control and arecoline‐treated groups. (D) Bar plots showing the quantification of migration and invasion assays for SCC25 and SCC9 cells under control and arecoline‐treated conditions ( n = 3, *** p < 0.001, **** p < 0.0001 by Student's t ‐test).
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Arecoline enhances the migratory and invasive potential of LAMC2 + OSCC cells. (A) Representative immunohistochemical staining images showing LAMC2 expression in OSCC tissues from betel nut chewers and nonchewers. The box‐and‐whisker plot on the right summarizes LAMC2 expression levels; each dot represents an individual clinical specimen (**** p < 0.0001 by Student's t ‐test). (B) Western blot images showing the expression of LAMC2, EMT‐associated markers (N‐cadherin, E‐cadherin, and Vimentin), and GAPDH in OSCC cell lines <t>SCC25</t> and SCC9 under control and arecoline‐treated conditions. (C) Representative Transwell assay images illustrating migratory and invasive capacities of SCC25 and SCC9 cells in control and arecoline‐treated groups. (D) Bar plots showing the quantification of migration and invasion assays for SCC25 and SCC9 cells under control and arecoline‐treated conditions ( n = 3, *** p < 0.001, **** p < 0.0001 by Student's t ‐test).
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Arecoline enhances the migratory and invasive potential of LAMC2 + OSCC cells. (A) Representative immunohistochemical staining images showing LAMC2 expression in OSCC tissues from betel nut chewers and nonchewers. The box‐and‐whisker plot on the right summarizes LAMC2 expression levels; each dot represents an individual clinical specimen (**** p < 0.0001 by Student's t ‐test). (B) Western blot images showing the expression of LAMC2, EMT‐associated markers (N‐cadherin, E‐cadherin, and Vimentin), and GAPDH in OSCC cell lines <t>SCC25</t> and SCC9 under control and arecoline‐treated conditions. (C) Representative Transwell assay images illustrating migratory and invasive capacities of SCC25 and SCC9 cells in control and arecoline‐treated groups. (D) Bar plots showing the quantification of migration and invasion assays for SCC25 and SCC9 cells under control and arecoline‐treated conditions ( n = 3, *** p < 0.001, **** p < 0.0001 by Student's t ‐test).
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Arecoline enhances the migratory and invasive potential of LAMC2 + OSCC cells. (A) Representative immunohistochemical staining images showing LAMC2 expression in OSCC tissues from betel nut chewers and nonchewers. The box‐and‐whisker plot on the right summarizes LAMC2 expression levels; each dot represents an individual clinical specimen (**** p < 0.0001 by Student's t ‐test). (B) Western blot images showing the expression of LAMC2, EMT‐associated markers (N‐cadherin, E‐cadherin, and Vimentin), and GAPDH in OSCC cell lines SCC25 and SCC9 under control and arecoline‐treated conditions. (C) Representative Transwell assay images illustrating migratory and invasive capacities of SCC25 and SCC9 cells in control and arecoline‐treated groups. (D) Bar plots showing the quantification of migration and invasion assays for SCC25 and SCC9 cells under control and arecoline‐treated conditions ( n = 3, *** p < 0.001, **** p < 0.0001 by Student's t ‐test).

Journal: MedComm

Article Title: Comprehensive Integrated Single‐Cell and Spatial Transcriptomics Unveil the Dynamic Landscape of Betel Nut‐Associated Oral Mucosal Carcinogenesis and Its Tumor Microenvironment

doi: 10.1002/mco2.70796

Figure Lengend Snippet: Arecoline enhances the migratory and invasive potential of LAMC2 + OSCC cells. (A) Representative immunohistochemical staining images showing LAMC2 expression in OSCC tissues from betel nut chewers and nonchewers. The box‐and‐whisker plot on the right summarizes LAMC2 expression levels; each dot represents an individual clinical specimen (**** p < 0.0001 by Student's t ‐test). (B) Western blot images showing the expression of LAMC2, EMT‐associated markers (N‐cadherin, E‐cadherin, and Vimentin), and GAPDH in OSCC cell lines SCC25 and SCC9 under control and arecoline‐treated conditions. (C) Representative Transwell assay images illustrating migratory and invasive capacities of SCC25 and SCC9 cells in control and arecoline‐treated groups. (D) Bar plots showing the quantification of migration and invasion assays for SCC25 and SCC9 cells under control and arecoline‐treated conditions ( n = 3, *** p < 0.001, **** p < 0.0001 by Student's t ‐test).

Article Snippet: SCC9 and SCC25 cells were obtained from the American Type Culture Collection.

Techniques: Immunohistochemical staining, Staining, Expressing, Whisker Assay, Western Blot, Control, Transwell Assay, Migration